The VioOne™ HIV Profile™ Supplemental Assay is an enzyme-linked immunosorbent assay (ELISA) for confirmation and differentiation of individual antibodies directed to various gene products of HIV-1 (Group M & Group O) and HIV-2 in human serum or plasma. It is a more specific test used to confirm the presence of HIV-1 and/or HIV-2 antibodies from specimens that were repeatedly reactive in diagnostic screening procedures.